Everything about 5-methylcytosine totally explained
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5-Methylcytosine is a
methylated form of
cytosine in which a
methyl group is attached to carbon 5, altering its structure without altering its base-pairing properties.
In vivo
5-Methylcytosine is an
epigenetic modification formed by the action of
DNA methyltransferases. Its function varies significantly among species:
- In bacteria, 5-methylcytosine can be found at a variety of sites, and is often used as a marker to protect DNA from being cut by native methylation-sensitive restriction enzymes.
- In plants, 5-methylcytosine occurs at CpG, CpNpG and CpNpN sequences (where N = A, C or T).
- In fungi and animals, 5-methylcytosine predominantly occurs at CpG dinucleotides. Although most eukaryotes methylate only a small percentage of these sites, in vertebrates 70-80% of CpG cytosines are methylated.
While spontaneous
deamination of cytosine forms uracil, which is recognized and removed by DNA repair enzymes, deamination of 5-methylcytosine forms
thymine. This conversion of a DNA base from cytosine (C) to thymine (T) can result in a
transition mutation.
In vitro
5-Methylcytosine can be deaminated to form thymine with use of reagents such as nitrous acid; cytosine deaminates to uracil under similar conditions.
5-Methylcytosine is resistant to deamination by bisulfite treatment, which deaminates cytosine residues; this property is often exploited to analyze DNA cytosine methylation patterns with
bisulfite sequencing.
Additional images
Image:Cytosine chemical structure.png |Cytosine
Further Information
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